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By J. Bignon
Read or Download Biochemistry, Pathology and Genetics of Pulmonary Emphysema. Proceedings of an International Symposium Held in Sassari, Italy, 27–30 April 1980 PDF
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Additional resources for Biochemistry, Pathology and Genetics of Pulmonary Emphysema. Proceedings of an International Symposium Held in Sassari, Italy, 27–30 April 1980
G o d f r e y (Suny-Stony Brook). Iodine-125 (Bolton-Hunter reagent) w a s from N e w England N u c l e a r (Boston, Massachusetts). Lysine w a s from M a n n Research Laboratories ( N e w York, N e w Y o r k ) . D e s m o s i n e and i s o d e s m o s i n e were obtained from Elastin Products (St. Louis, Missouri). U s i n g c o m m e r c i a l d e s m o s i n e as starting material, chromatographically-pure d e s m o s i n e w a s prepared according to the m e t h o d of Y u et al.  and the purified desmosine-cont^ining fractions were c o m b i ned and lyophilized.
C o r r e s p o n d e n c e : S. HAREL (C/O A . Sciences Center, State U n i v e r s i t y of N e w Y o r k USA. M e d i c i n e , State U n i v e r s i t y of N e w Y o r k at V e t e r a n s A d m i n i s t r a t i o n Hospital, St. L o u i s , JANOFF), D e p a r t m e n t of P a t h o l o g y , H e a l t h at S t o n y Brook, S t o n y Brook, N . Y . 1 1 7 9 4 , 76 S. HAREL ET AL. symposium, p. 6 5 ) , anti-peptide antisera have so far not been successfully applied to the detection of low concentrations of elastin breakdown products in diseased humans.
Darnule, A T . Darnule, I. Mandl A B S T R A C T : A n t i g e n i c determinants of h u m a n l u n g elastin peptides w e r e studied b y fractionation o n an S E - S e p h a d e x C - 5 0 c o l u m n . B y elution profile, tubes w e r e p o o l e d into 12 fractions. A m i n o acid analysis of these fractions s h o w e d that the first s e v e n c o n t a i n e d m o s t l y non-polar peptides w h i c h lacked d e s m o s i n e and i s o d e s m o s i n e residues. T h e other five fractions contained m o r e polar peptides with d e s m o s i n e and i s o d e s m o s i n e .