Download Biochemistry, Pathology and Genetics of Pulmonary Emphysema. by J. Bignon PDF
By J. Bignon
Read or Download Biochemistry, Pathology and Genetics of Pulmonary Emphysema. Proceedings of an International Symposium Held in Sassari, Italy, 27–30 April 1980 PDF
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Additional resources for Biochemistry, Pathology and Genetics of Pulmonary Emphysema. Proceedings of an International Symposium Held in Sassari, Italy, 27–30 April 1980
Sample text
G o d f r e y (Suny-Stony Brook). Iodine-125 (Bolton-Hunter reagent) w a s from N e w England N u c l e a r (Boston, Massachusetts). Lysine w a s from M a n n Research Laboratories ( N e w York, N e w Y o r k ) . D e s m o s i n e and i s o d e s m o s i n e were obtained from Elastin Products (St. Louis, Missouri). U s i n g c o m m e r c i a l d e s m o s i n e as starting material, chromatographically-pure d e s m o s i n e w a s prepared according to the m e t h o d of Y u et al. [17] and the purified desmosine-cont^ining fractions were c o m b i ned and lyophilized.
C o r r e s p o n d e n c e : S. HAREL (C/O A . Sciences Center, State U n i v e r s i t y of N e w Y o r k USA. M e d i c i n e , State U n i v e r s i t y of N e w Y o r k at V e t e r a n s A d m i n i s t r a t i o n Hospital, St. L o u i s , JANOFF), D e p a r t m e n t of P a t h o l o g y , H e a l t h at S t o n y Brook, S t o n y Brook, N . Y . 1 1 7 9 4 , 76 S. HAREL ET AL. symposium, p. 6 5 ) , anti-peptide antisera have so far not been successfully applied to the detection of low concentrations of elastin breakdown products in diseased humans.
Darnule, A T . Darnule, I. Mandl A B S T R A C T : A n t i g e n i c determinants of h u m a n l u n g elastin peptides w e r e studied b y fractionation o n an S E - S e p h a d e x C - 5 0 c o l u m n . B y elution profile, tubes w e r e p o o l e d into 12 fractions. A m i n o acid analysis of these fractions s h o w e d that the first s e v e n c o n t a i n e d m o s t l y non-polar peptides w h i c h lacked d e s m o s i n e and i s o d e s m o s i n e residues. T h e other five fractions contained m o r e polar peptides with d e s m o s i n e and i s o d e s m o s i n e .